ASTM-E1285 › Standard Guide for Identification of Bacteriophage Lambda (λ) or Its DNA (Withdrawn 2014)
The following bibliographic material is provided to assist you with your purchasing decision:
This guide covers laboratory characterization procedures for identifying bacteriophage lambda or its DNA and assumes that the reader has basic knowledge in virology and molecular biology. Bacteriohage lambda is a temperate bacteriophage with an icosahedral hear and a single, non-contractile tail ending in a single tail fiber. The lambda genome consists of a single molecule of linear double-stranded DNA and has cohesive ends. The naturally preferred hosts is Escherichia coli K12. Hundreds of lambda variants derived from wild type lambda can be used in biotechnology and differ in genome size and genotype. These are used primarily as DNA vectors for cloning DNA fragments. Judging uncontaminated, pure lambda should be done through restriction enzyme analysis DNA characterization and the presence and identification of lambda DNA is accomplished by polymerase chain reaction. The primers used for detection of bacteriophage lambda should be chosen based on the reason for detection.
Scope
1.1 This guide covers the procedures for identifying bacteriophage lambda used in biotechnology.
1.2 There are hundreds of lambda variants that can be used for biotechnology. These lambda variants are derived from wild type lambda and differ in genome size and genotype.
1.3 If the bacteriophage lambda is to be used to construct a recombinant molecule, then the same criteria as prescribed in Section 5 should be used to characterize the newly made DNA.
Keywords
bacteriophage; cloning vector; lambda; PCR; polymerase chain reaction; recombinant DNA; ICS Number Code 07.100.10 (Medical microbiology)
To find similar documents by ASTM Volume:
11.06 (Biological Effects and Environmental Fate; Biotechnology)
To find similar documents by classification:
07.100.10 (Medical microbiology Laboratory medicine, see 11.100)
This document comes with our free Notification Service, good for the life of the document.
This document is available in either Paper or PDF format.
Document Number
ASTM-E1285-06
Revision Level
2006 EDITION
Status
Cancelled
Modification Type
Withdrawn
Publication Date
Jan. 1, 2006
Document Type
Guide
Page Count
2 pages
Committee Number
E55.04