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This standard establishes the principles and basic methodology for microbiological control of cleanrooms and associated controlled environments with planetary protection constraints. This standard does not address: • the microbiological contamination control of spaceflight hardware; • molecular contamination control. Reference is made to other documents; • fire and safety regulations; for these, see regulatory requirements and other national or local documentation. This standard does not lay down the methods for determining the microbiological and particulate cleanliness levels. Reference is made to other documents. This standard may be tailored for the specific characteristic and constrains of a space project in conformance with ECSS-S-ST-00.