SS-EN-ISO-19040-1 › Water quality - Determination of the estrogenic potential of water and waste water - Part 1: Yeast estrogen screen (Saccharomyces cerevisiae) (ISO 19040-1:2018)
SS-EN-ISO-19040-1
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2022 EDITION
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CURRENT
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This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with genetically modified yeast strains Saccharomyces cerevisiae. This reporter gene assay is based on the activation of the human estrogen receptor alpha. This method is applicable to: fresh water; waste water; aqueous extracts and leachates; eluates of sediments (fresh water); pore water; aqueous solutions of single substances or of chemical mixtures; drinking water. The limit of quantification (LOQ) of this method for the direct analysis of water samples is between 8 ng/l and 15 ng/l 17ß-estradiol equivalents (EEQ) based on the results of the international interlaboratory trial (see Annex F). The upper threshold of the dynamic range for this test is between 120 ng/l and 160 ng/l 17ß-estradiol equivalents (EEQ). Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre-concentration of water samples can prove necessary, if their estrogenic potential is below the given LOQ.
To find similar documents by classification:
13.060.70 (Examination of biological properties of water Microbiology of water, see 07.100.20)
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Designation Name
SS-EN ISO 19040-1:2022
Revision Level
2022 EDITION
Status
Current
Publication Date
Sept. 28, 2022
Language(s)
English
Page Count
64
International Equivalent
EN ISO 19040-1:2022(IDT); ISO 19040-1:2018(IDT)