SS-EN-ISO-23418 › Microbiology of the food chain - Whole genome sequencing for typing and genomic characterization of bacteria - General requirements and guidance (ISO 23418:2022)
SS-EN-ISO-23418
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2022 EDITION
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CURRENT
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This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages: a) handling of bacterial cultures; b) axenic genomic DNA isolation; c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage; d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation; e) metadata capture and sequence repository deposition; f) validation of the end-to-end WGS workflow (fit for purpose for intended application). This document is applicable to bacteria isolated from: products intended for human consumption; products intended for animal feed; environmental samples from food and feed handling and production areas; samples from the primary production stage.
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Designation Name
SS-EN ISO 23418:2022
Revision Level
2022 EDITION
Status
Current
Publication Date
June 28, 2022
Language(s)
English
Page Count
56
International Equivalent
EN ISO 23418:2022(IDT); ISO 23418:2022(IDT)