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This document provides methods for — calibrating an instrument with one or two calibration gas mixtures, — determining the composition of a gas sample, and — evaluating the uncertainty of the composition of the gas sample in relation to the uncertainty of the composition of the calibration gases used and the contribution of the measurement process. This document sets requirements to, and acceptance criteria for, the utilization of different measurement calibration designs with a limited (i.e. minimum) number of calibration gas mixtures used in calibration. The methods in this document are described for amount-of-substance fractions, but are also applicable for other composition quantities (such as mass fractions, volume fractions or concentrations).