ASTM-D4133 Standard Practice for Sampling Phytoplankton with Pumps (Withdrawn 2020)

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Standard Practice for Sampling Phytoplankton with Pumps (Withdrawn 2020)


1.1 This practice covers the procedures for obtaining qualitative samples of a phytoplankton community by use of pumping systems.

1.2 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Significance and Use

4.1 The advantages of a pumping phytoplankton sampler are as follows:

4.1.1 Sample size is more accurately controlled than with the use of a conical tow net.

4.1.2 Discrete samples from any depth are easily obtained.

4.1.3 Multiple or replicate samples are easily obtained.

4.1.4 They are adaptable to shallow ecosystems.

4.1.5 They allow for a stable collecting efficiency.

4.2 The disadvantages of a pumping phytoplankton sampler are as follows:

4.2.1 They generally are bulky and frequently require an electrical source.

4.2.2 They generally are costly.

4.2.3 They collect only qualitative samples, or semiquantitative samples when used with a volume register.

4.3 There are several special considerations that shall be observed when using a pumping phytoplankton sampler. They are as follows:

4.3.1 Pumps can induce mortality of the organisms and damage the delicate forms.

4.3.2 If tubing is not properly cleaned after use, contamination of subsequent samples can ensue.

To find similar documents by ASTM Volume:

11.02 (Water (II))

11.05 (Pesticides and Alternative Control Agents; Environmental Assessment; Hazardous Substances and Oil Spill Response)

11.06 (Biological Effects and Environmental Fate; Biotechnology)

To find similar documents by classification:

07.100.20 (Microbiology of water Examination of biological properties of water, see 13.060.70)

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Document Number


Revision Level

1982 R12 EDITION



Modification Type


Publication Date

Oct. 15, 2012

Document Type


Page Count

2 pages

Committee Number