ASTM-D4454 Standard Test Method for Simultaneous Enumeration of Total and Respiring Bacteria in Aquatic Systems by Microscopy (Withdrawn 2015)

ASTM-D4454 - 1985 R09 EDITION - SUPERSEDED
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Standard Test Method for Simultaneous Enumeration of Total and Respiring Bacteria in Aquatic Systems by Microscopy (Withdrawn 2015)
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Scope

1.1 This test method covers the detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. This test method is applicable to environmental waters and potable waters.

1.2 Certain types of debris and other microorganisms may fluoresce in acridine-orange stained smears.

1.3 The procedure described requires a trained microbiologist or technician who is capable of distinguishing bacteria from other fluorescing bodies on the basis of morphology when viewed at higher magnifications.

1.4 Use of bright light permits differentiation of single bacteria where reduced formazan is deposited at the polar ends.

1.5 Approximately 104 cells/mL are required for detection by this test method.

1.6 Minimal cell size which allows the detection of formazan deposits is represented by bacteria of 0.4 μm.2

1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.8 This standard does not purport to address the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Keywords

Water-formed deposits; Bacteria/bacterial control--water; Epifluorescence microscopy; Fluorescence--water analysis; Microscopic examination--water; Pathogens--water; ICS Number Code 07.100.20 (Microbiology of water)

To find similar documents by ASTM Volume:

11.02 (Water (II))

To find similar documents by classification:

07.100.20 (Microbiology of water Examination of biological properties of water, see 13.060.70)

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Document Number

ASTM-D4454-85(2009)

Revision Level

1985 R09 EDITION

Status

Superseded

Modification Type

Withdrawn

Publication Date

July 1, 2009

Document Type

Test Method

Page Count

3 pages

Committee Number

D19.24