ASTM-D5244 Standard Practice for Recovery of Enteroviruses from Waters (Withdrawn 2013)

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Standard Practice for Recovery of Enteroviruses from Waters (Withdrawn 2013)


1.1 This practice covers a uniform procedure for the concentration of viruses from collected samples.

1.2 This practice describes a virus adsorption-elution cartridge filter procedure for recovering viruses from drinking water. Volumes of 400 L or more are processed for samples of drinking water quality.

1.3 The principles of this practice are also applicable to sewages, effluents, and surface waters without technical modifications.

1.4 Although specifically designed for recovery of human enteroviruses, this practice also may be applied to some other human enteric viruses, that have to be determined by specific testing.

1.5 The consistency of this practice was determined from method evaluation studies with poliovirus-seeded drinking water samples.

1.6 The values stated in SI units are to be regarded as the standard.

1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Only adequately trained personnel should be allowed to perform these procedures and should use safety precautions recommended by the U.S. Public Health Service Center for Disease Control for work with potentially hazardous biological organisms.

Significance and Use

Enteric viruses of public health significance are present in the aquatic environment.

Enteric viruses have been detected in treated water supplies.

Enteric viruses are responsible for a wide range of illnesses, ranging from hepatitis to gastroenteritis.

This practice is applicable to the recovery of many plaque-forming enteric viruses from waters when used in conjunction with cell culture assay systems.

The principles of this practice are applicable without technical modifications for monitoring for viruses based on the use of gene probe technology.


Adsorption; cartridge filter; concentration; elution; enteric viruses; enteroviruses; human pathogens; water monitoring; water quality; ICS Number Code 07.100.20 (Microbiology of water)

To find similar documents by ASTM Volume:

11.02 (Water (II))

To find similar documents by classification:

07.100.20 (Microbiology of water Examination of biological properties of water, see 13.060.70)

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Document Number


Revision Level

1992 R04 EDITION



Modification Type


Publication Date

May 1, 2004

Document Type


Page Count

3 pages

Committee Number