ASTM-D5465 › Standard Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
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1.1 These practices cover recommended procedures for counting colonies and reporting colony-forming units (CFU) on membrane filters (MF) and standard pour and spread plates.
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
Significance and Use
4.1 Numerous ASTM test methods and practices (for example: Test Methods and , and Practices and ) report colony counts as their measured parameter.
4.2 These practices provide a uniform set of counting, calculating, and reporting procedures for ASTM test methods in microbiology.
A—Counting Colonies on Membrane Filters
B—Counting Colonies on Pour Plates
C—Counting Colonies on Spread Plates
4.3 The counting rules provide a best attainable estimate of microorganisms in the sample, since the samples cannot be held and reanalyzed at a later date.
calculating results; counting colonies; membrane filter methods; pour plate methods; reporting results; rounding off; significant figures; spread plate methods; verification of counts ;; ICS Number Code 07.100.20 (Microbiology of water)
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July 1, 2016