ASTM-D5810 › Standard Guide for Spiking into Aqueous Samples
The following bibliographic material is provided to assist you with your purchasing decision:
Scope
1.1 This guide covers the general technique of "spiking" a broad range of materials into aqueous media. This guide will serve the analyst in preparing spiked samples for quality control purposes. Guidance is also provided to aid the analyst in calculating recoveries and interpreting results. It is the responsibility of the analyst to determine whether the procedures and materials described here are appropriate to the task at hand.
1.2 The procedures in this guide are focused on "matrix spike" preparation, analysis, and interpretation of results. The applicability of these procedures to the preparation of calibration standards, calibration check standards, laboratory control standards, reference materials, and other quality control materials by spiking is incidental. A sample (the matrix) is fortified (spiked) with the analyte of interest for a variety of analytical and quality control purposes. While the spiking of multiple sample portions is discussed, the method of standard additions is not covered.
1.3 This guide is intended for use in conjunction with the individual analytical test method that provides procedures for analysis of the analyte or component of interest. The test method is used to determine an analyte or component's background level and, again after spiking, its now elevated level. Each test method typically provides procedures not only for samples, but also for calibration standards or analytical control solutions, or both. These procedures include preparation, handling, storage, preservation, and analysis techniques. These procedures are applicable by extension, using the analyst's judgement on a case-by-case basis, to spiking solutions, and are not reiterated in this guide. See also Practice E200 for preparation and storage information.
1.4 These procedures apply only to analytes that are soluble in water at the concentration of the spike plus any background material, or to analytes soluble in a solvent that is itself water-soluble. The system used in the later case must result in a homogeneous solution of analyte and sample. Meaningful recovery data cannot be obtained if an aqueous solution or homogenous suspension of the analyte of interest in the sample cannot be attained. These procedures may be applicable to microbiological preparations if the homogeneity of the suspension can be adequately maintained throughout the course of the analysis, for example, by mechanical agitation or stirring.
1.5 Matrix spiking may be performed in the field or in the laboratory, depending on which part of the analytical process is to be tested. Field spiking tests the recovery of the overall process, including preservation and shipping of the sample. Laboratory spiking tests the laboratory process only. Spiking of sample extracts, concentrates, or dilutions will test only that portion of the process subsequent to addition of the spike.
1.6 The values stated in SI units are to be regarded as the standard.
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
Keywords
bias; internal standards; matrix spike; percent recovery; quality assurance; recovery; spike; standard additions; surrogates
To find similar documents by ASTM Volume:
To find similar documents by classification:
19.020 (Test conditions and procedures in general)
This document comes with our free Notification Service, good for the life of the document.
This document is available in either Paper or PDF format.
Customers who bought this document also bought:
ASTM-E8Standard Test Methods for Tension Testing of Metallic Materials
ASTM-E4
Standard Practices for Force Verification of Testing Machines
ASTM-F519
Standard Test Method for Mechanical Hydrogen Embrittlement Evaluation of Plating/Coating Processes and Service Environments
Document Number
ASTM-D5810-96R21
Revision Level
1996 R21 EDITION
Status
Current
Modification Type
Revision
Publication Date
July 16, 2021
Document Type
Guide
Page Count
6 pages
Committee Number
D19.02