ASTM-D6974 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures

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Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures



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1.1 This practice covers a membrane filter (MF) procedure for the detection and enumeration of Heterotrophic bacteria (HPC) and fungi in liquid fuels with kinematic viscosities 24 mm2 · s-1 at ambient temperature.

1.2 This quantitative practice is drawn largely from IP Method 385 and Test Method D5259.

1.3 This test may be performed either in the field or in the laboratory.

1.4 The ability of individual microbes to form colonies on specific growth media depends on the taxonomy and physiological state of the microbes to be enumerated, the chemistry of the growth medium, and incubation conditions. Consequently, test results should not be interpreted as absolute values. Rather they should be used as part of a diagnostic or condition monitoring effort that includes other test parameters, in accordance with Guide D6469.

1.5 This practice offers alternative options for delivering fuel sample microbes to the filter membrane, volumes or dilutions filtered, growth media used to cultivate fuel-borne microbes, and incubation temperatures. This flexibility is offered to facilitate diagnostic efforts. When this practice is used as part of a condition monitoring program, a single procedure should be used consistently.

1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


bacteria; biodegradation; biodeterioration; colony forming unit; enumeration; fuel microbiology; fungi; membrane filter technique; viable count;; ICS Number Code 75.160.20 (Liquid fuels)

To find similar documents by ASTM Volume:

05.04 (Petroleum Products and Lubricants (IV): D6973 - latest)

To find similar documents by classification:

75.160.20 (Liquid fuels Including gasoline, diesel, kerosene, etc.)

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Publication Date

July 15, 2016

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5 pages

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