ASTM-E1286 Standard Guide for Identification of Herpes Simplex Virus or Its DNA (Withdrawn 2014)

ASTM-E1286 - 2000 R06 EDITION - SUPERSEDED
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Standard Guide for Identification of Herpes Simplex Virus or Its DNA (Withdrawn 2014)

Scope

1.1 This guide covers laboratory characterization procedures sufficient to identify purified specimens of HSV types 1 and 2 (HSV-1 and HSV-2) or HSV-1 DNA and HSV-2 DNA used in biotechnology. For cases in which identification of HSV DNA specimens is required, the characterization criteria of and of this guide are sufficient.

1.2 This guide does not cover the identification of HSV in HSV-infected host cells. To apply this guide to such a case, it would first be necessary to isolate the virus from such samples using standard techniques of HSV purification. This guide does not cover characterization of segments of HSV DNA or of vectors containing HSV DNA segments.

1.3 This guide does not cover the specific methodology used in the identification characterization. It does not address the question of degree of purity required for herpes virus preparations: this would vary depending on the particular biotechnology use of the virus.

1.4 Warning-Laboratory work involving herpes simplex viruses can be hazardous to personnel. Biosafety 2 level facilities are recommended (). Safety guidelines shall be adhered to according to NCCLS M29-T2 and other recommendations ().

This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Significance and Use

This guide is intended for use in a biotechnology laboratory whenever the necessity arises for identifying a biological preparation believed to contain primarily HSV or its DNA. The characterization criteria used for the identification shall be performed by an individual trained in molecular virology.

This guide is not meant to be used in a clinical laboratory for the identification of HSV isolated from patient specimens.

Keywords

ICS Number Code 07.100.10 (Medical microbiology)

To find similar documents by ASTM Volume:

11.06 (Biological Effects and Environmental Fate; Biotechnology)

14.02 (General Test Methods; Forensic Psychophysiology; Forensic Sciences; Terminology; Conformity Assessment; Statistical Methods; Nanotechnology; Forensic Engineering; Manufacture of Pharmaceutical Products)

To find similar documents by classification:

07.100.10 (Medical microbiology Laboratory medicine, see 11.100)

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Document Number

ASTM-E1286-00(2006)

Revision Level

2000 R06 EDITION

Status

Superseded

Modification Type

Withdrawn

Publication Date

Jan. 1, 2006

Document Type

Guide

Page Count

3 pages

Committee Number

E55.04