ASTM-E1536 › Standard Practice for Detection of Mycoplasma Contamination of Bovine Serum by Large Volume Method (Withdrawn 2014)
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1.1 This practice covers the procedures used for detection of mycoplasma contamination in serum by direct microbiological culture.
1.2 This practice does not cover procedures used for detection of mycoplasma in cell cultures.
1.3 This practice does not cover indirect methods for detection of mycoplasma contamination.
1.4 This practice does not cover methods for identification of mycoplasma cultures.
This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
Significance and Use
Heat inactivated serum need not be tested for mycoplasmas. Heating serum to 56°C for 30 min will kill mycoplasmas.
Mycoplasmas may be present in any particular lot of serum but may not be detected because of inadequate sample size; thus, negative test results do not provide absolute assurance that the test serum is free of mycoplasmas.
mycoplasma; serum; ICS Number Code 07.100.10 (Medical microbiology)
To find similar documents by ASTM Volume:
14.02 (General Test Methods; Forensic Psychophysiology; Forensic Sciences; Terminology; Conformity Assessment; Statistical Methods; Nanotechnology; Forensic Engineering; Manufacture of Pharmaceutical Products)
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2000 R06 EDITION
Jan. 1, 2006