ASTM-E1874 Standard Test Method for Recovery of Microorganisms From Skin using the Cup Scrub Technique

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Standard Test Method for Recovery of Microorganisms From Skin using the Cup Scrub Technique



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1.1 This test method is designed to recover microorganisms from the skin of human subjects or human subject surrogates (animal skin, isolated porcine skin, human skin equivalents, and other such surfaces).

1.2 Knowledge of microbiological techniques is required for these procedures.

1.3 It is the responsibility of the investigator to determine if Good Laboratory Practice (GLP) and Good Clinical Practice (GCP) is required.

1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Significance and Use

5.1 The procedure can be incorporated into protocols used to evaluate test materials containing antibacterial ingredients that are intended to reduce significantly the number of organisms on intact skin. It also may be used to provide an indication of residual antibacterial activity. Examples of test materials, for which this method is applicable, include hand-washes, surgical scrubs, acne reduction products, and others. For each type of test material, types of resident flora or transient organisms, or a combination thereof, may differ and should be considered (this is, aerobic bacteria, anaerobic bacteria, yeast, or mold).

5.2 The procedure may be used in protocols intended to evaluate and identify resident flora from the skin.

5.3 Performance of this technique may require the knowledge of regulations pertaining to the protection of human subjects if the protocol involves application of the technique to the skin of human subjects.


cup scrub; resident flora; skin; skin equivalent; transient organism; ICS Number Code 71.100.40 (Surface active agents)

To find similar documents by ASTM Volume:

11.05 (Pesticides and Alternative Control Agents; Environmental Assessment; Hazardous Substances and Oil Spill Response)

To find similar documents by classification:

71.100.40 (Surface active agents)

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Publication Date

April 15, 2014

Document Type

Test Method

Page Count

3 pages

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