ASTM-E2563 Standard Practice for Enumeration of Non-Tuberculosis Mycobacteria in Aqueous Metalworking Fluids by Plate Count Method

ASTM-E2563 - 2013 EDITION - CURRENT
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Standard Practice for Enumeration of Non-Tuberculosis Mycobacteria in Aqueous Metalworking Fluids by Plate Count Method
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Scope

1.1 This practice covers the detection and enumeration of viable and culturable rapidly growing Mycobacteria (RGM), or non-tuberculosis Mycobacteria (NTM) in aqueous metalworking fluids (MWF) in the presence of high non-mycobacterial background population using standard microbiological culture methods.

1.2 The detection limit is one colony forming unit (CFU)/mL metalworking fluid.

1.3 This practice involves culture of organisms classified as Level 2 pathogens, and should be undertaken by a trained microbiologist in an appropriately equipped facility. The microbiologist should also be capable of distinguishing the diverse colonies of Mycobacteria from other microorganism colonies on a Petri dish and capable of confirming Mycobacteria by acid fast staining method

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Keywords

acid-fast bacteria; hypersensitivity pneumonitis; metalworking fluid; non-tuberculous Mycobacteria; rapidly growing Mycobacteria; ICS Number Code 75.100 (Lubricants, industrial oils and related products)

To find similar documents by ASTM Volume:

11.03 (Occupational Health and Safety; Protective Clothing)

To find similar documents by classification:

75.100 (Lubricants, industrial oils and related products Including mineral oils, fluids for metal working and for temporary protection against corrosion Lubrication systems, see 21.260 Insulating oils, see 29.035.40)

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Document Number

ASTM-E2563-13

Revision Level

2013 EDITION

Status

Current

Modification Type

Revision with Title Change

Publication Date

July 15, 2013

Document Type

Practice

Page Count

3 pages

Committee Number

E34.50