ASTM-E2564 › Standard Practice for Enumeration of
Mycobacteria in Metalworking Fluids by Direct Microscopic Counting (DMC) Method
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1.1 This practice describes a direct microscopic counting method (DMC) for the enumeration of the acid fast stained mycobacteria population in metalworking fluids. It can be used to detect levels of total mycobacteria population, including culturable as well as non-culturable (possibly dead or moribund ) bacterial cells. This practice is recommended for all water-based metalworking fluids.
1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For additional safety information, see Laboratory Safety: Principle and Practices, 4th Edition2
acid-fast Bacteria (AFB); acid-fast staining; direct microscopic count (DMC); hypersensitivity pneumonitis (HP) ; metalworking fluid (MWF); microscopic factor (MF); non-tuberculous mycobacterium (NTM); ICS Number Code 07.100.99 (Other standards related to microbiology); 75.100 (Lubricants, industrial oils and related products)
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75.100 (Lubricants, industrial oils and related products Including mineral oils, fluids for metal working and for temporary protection against corrosion Lubrication systems, see 21.260 Insulating oils, see 29.035.40)
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Revision with Title Change
July 15, 2013