ASTM-E2839 › Standard Test Method for Production of Clostridium difficile Spores for Use in Efficacy Evaluation of Antimicrobial Agents
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1.1 This test method is for producing C. difficile spores to evaluate antimicrobial formulations for their sporicidal activity.
1.2 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP) are required and to follow them when appropriate.
1.3 This standard may involve hazardous materials, chemicals, and microorganisms and should be performed only by persons with formal training in microbiology.
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
acid resistance; Clostridium difficile; density gradient medium; spore production; spore purity; sporicidal efficacy testing; vegetative cells; ICS Number Code 07.100.99 (Other standards related to microbiology)
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Sept. 15, 2011