ASTM-E2894 Standard Test Method for Applying Aerosolized Bacillus Spores as Dry Inocula to Inanimate Surfaces

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Standard Test Method for Applying Aerosolized Bacillus Spores as Dry Inocula to Inanimate Surfaces



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1.1 This test method is designed to uniformly apply fluidized spores to surfaces as an aerosol under defined conditions.

1.2 This test method is specific to B. anthracis Delta (Δ)Sterne (BAΔS), but could be adapted for work with other types of Bacillus species.

1.3 This test method is suitable for working with any type of environmental surface.

1.4 This test method should be performed only by those trained in aerobiology, microbiology, or a combination thereof.

1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


aerosols; anthrax; antimicrobial; Bacillus; bioaerosol; chambers; deposition; extraction; infectious bioagents; spores; ICS Number Code 07.100.01 (Microbiology in general)

To find similar documents by ASTM Volume:

11.05 (Pesticides and Alternative Control Agents; Environmental Assessment; Hazardous Substances and Oil Spill Response)

To find similar documents by classification:

07.100.01 (Microbiology in general)

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Publication Date

March 15, 2014

Document Type

Test Method

Page Count

7 pages

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