ISO-19056-3 Microscopes - Definition and measurement of illumination properties - Part 3: Incident light fluorescence microscopy with incoherent light sources

ISO-19056-3 - 1ST EDITION - CURRENT


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This document specifies procedures for the measurement of illumination brightness, temporal stability and uniformity for incident light fluorescence microscopy. The measurement for uniformity is defined in image planes or intermediate image planes only, when these planes are suitable for detection by electronic imaging devices.

This document defines how illumination brightness, temporal stability and uniformity are measured, and how this information is provided to the user.

NOTE      The scope is intentionally limited to electronic imaging devices and (intermediate) image planes. The visual observation by means of eyepieces would require a different measurement procedure and hence result in ambiguities in the description of measurement procedures. Nevertheless, this document will give useful estimates for the uniformity with visual observation as in this case an eyepiece is used to observe an intermediate image plane (which is under the scope of this document).

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37.020 (Optical equipment Including microscopes, telescopes, binoculars, optical materials, optical components and optical systems Ophthalmic equipment, see 11.040.70 Optical measuring instruments, see 17.180.30 Photographic equipment lenses, see 37.040.10)

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Document Number

ISO 19056-3:2022

Revision Level

1ST EDITION

Status

Current

Publication Date

Jan. 1, 2022

Committee Number

ISO/TC 172/SC 5