ISO-21446 Infant formula and adult nutritionals - Determination of trans and total (cis + trans) vitamin K1 content - Normal phase HPLC

ISO-21446 - 1ST EDITION - CURRENT


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This document specifies a method for the quantitative determination of trans and total (cis + trans) vitamin K1 in infant, pediatric and adult nutritionals using normal phase (NP) high-performance liquid chromatography (HPLC) with post-column reduction and fluorescence detection. The method demonstrated good linearity over a standard range of ~2 μg/l to 80 μg/l trans vitamin K1, and the limit of quantification (LOQ) was estimated to be 0,4 μg/l for standards and 0,09 μg/100 g ready to feed (RTF) for samples assuming 4 grams of sample are diluted to 10 ml.

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67.050 (General methods of tests and analysis for food products Food microbiology, see 07.100.30 Sensory analysis, see 67.240)

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Document Number

ISO 21446:2019

Revision Level

1ST EDITION

Status

Current

Publication Date

Jan. 1, 2019

Committee Number

ISO/TC 34