ISO-23036-2 Microbiology of the food chain - Methods for the detection of Anisakidae L3 larvae in fish and fishery products - Part 2: Artificial digestion method

ISO-23036-2 - 1ST EDITION - CURRENT


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This document specifies a method for the detection of Anisakidae L3 larvae commonly found in marine and anadromous fishes. The method is applicable to fresh fish and/or frozen fish, as well as lightly processed fish products, such as marinated, salted or smoked. It is also suitable for visceral organs as a confirmatory method for a visual inspection scheme.

The artificial digestion method[4][5][6] is applicable to quantifying parasitic infections by estimating the number of parasites in the fish musculature and, when applied to fresh fish or lightly processed fish products (never frozen before processing), determining the viability of Anisakidae L3, which can be present.

This method does not apply to determining the species or genotype of detected parasites. Final identification is made by morphological and/or molecular methods.

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07.100.30 (Food microbiology Including microbiology of animal feeding stuffs Animal feeding stuffs, see 65.120 General methods of tests and analysis for food products, see 67.050)

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Document Number

ISO 23036-2:2021

Revision Level

1ST EDITION

Status

Current

Publication Date

April 1, 2021

Committee Number

ISO/TC 34/SC 9