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ISO 27065 establishes minimum performance, classification, and marking requirements for protective clothing worn by operators handling pesticide products as well as re-entry workers. For the purpose of ISO 27065, the term pesticide applies to insecticides, herbicides, fungicides, and other substances applied in liquid form that are intended to prevent, destroy, repel, or reduce any pest or weeds in agricultural settings, green spaces, roadsides, etc. It does not include biocidal products used for agricultural and non-agricultural settings.

Pesticide handling includes mixing and loading, application, and other activities such as cleaning contaminated equipment and containers. Concentrated pesticides are typically handled during mixing and loading. Protective clothing covered by ISO 27065includes, but is not limited to, shirts, jackets, trousers, coveralls, aprons, protective sleeves, caps/hats and other headwear (excluding hard hats made of rigid materials, e.g. hats worn by construction workers), and accessories used under knapsack/backpack sprayers.

ISO 27065 does not address items used for the protection of the respiratory tract, hands, and feet. ISO 27065 does not address protection against fumigants.