ISO-5510 Animal feeding stuffs - Determination of available lysine

ISO-5510 - 1ST EDITION - CURRENT


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The method consists in hydrolysing with hydrochloric acid of a ground test portion, dollowed by separation of the total lysine by column chromatography and determination by spectrometry at 570 nm. Reaction of a second ground test portion with an ethanolic solution of 2,4-dinitrofluorobenzene in an alkaline medium, purification, then hydrolysis with hydrochloric acid, separation of the non-available lysine by column chromatography, and determination by spectrometry at 570 nm.

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65.120 (Animal feeding stuffs Microbiology of animal feeding stuffs, see 07.100.30)

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Document Number

ISO 5510:1984

Revision Level

1ST EDITION

Status

Current

Publication Date

Nov. 15, 1984

Committee Number

ISO TC 34/SC 10