ISO-6888-1 Microbiology of the food chain - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 1: Method using Baird-Parker agar medium

ISO-6888-1 - 2ND EDITION - CURRENT -- See the following: ISO-6888-1-1
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 2ND EDITION - Aug. 1, 2021
 FOR 2ND EDITION AMENDMENT 1 SEE - Sept. 1, 2023

This document specifies a horizontal method for the enumeration of coagulase-positive staphylococci by counting the colonies obtained on a solid medium (Baird-Parker medium)[10] after aerobic incubation at 34 °C to 38 °C and coagulase confirmation.

This document is applicable to:

â..    products intended for human consumption;

â..    products intended for animal feeding;

â..    environmental samples in the area of food and feed production, handling, and

â..    samples from the primary production stage.

This horizontal method was originally developed for the examination of all samples belonging to the food chain.

Because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.

Based on the information available at the time of publication of this document, this method is not considered to be (fully) suited to the examination of fermented products or other products containing technological flora based on Staphylococcus spp (e.g. S. xylosus) (such as cheeses made from raw milk and certain raw meat products) likely to be contaminated by:

â..    staphylococci forming atypical colonies on a Baird-Parker agar medium;

â..    background flora that can obscure the colonies being sought.

Nevertheless, both this document and ISO 6888-2 are given equivalent status.

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07.100.30 (Food microbiology Including microbiology of animal feeding stuffs Animal feeding stuffs, see 65.120 General methods of tests and analysis for food products, see 67.050)

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Document Number

ISO 6888-1:2021

Revision Level

2ND EDITION

Status

Current

Publication Date

Aug. 1, 2021

Committee Number

ISO/TC 34/SC 9