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This part of ISO/TS 21268 specifies a test, which is aimed at determining the leaching behaviour of inorganic and organic constituents from a soil and soil material. The method is a once-through percolation test with water (0,001 mol/l CaCl2) under standardized conditions of flow rate. The material is leached under dynamic hydraulic conditions. The eluates obtained can be used to determine the ecological properties of the soil with respect to micro-organisms, flora and fauna. The test results enable the distinction between different release patterns, for instance wash-out and release under the influence of interaction with the matrix, when approaching local equilibrium between material and leachant. This test method produces eluates, which can subsequently be characterised by physical, chemical and ecotoxicological methods in accordance with existing standard methods. The results of eluate analysis are presented as a function of the liquid/solid ratio. The test is not suitable for species that are volatile under ambient conditions. NOTE 1 Volatile organic constituents include the low-molecular-weight components in mixtures such as mineral oil. NOTE 2 It is not always possible to optimise test conditions simultaneously for inorganic and organic constituents andoptimum test conditions may also vary between different groups of organic constituents. Test requirements for organic constituents are generally more stringent than those for inorganic constituents. The test conditions suitable for measuring the release of organic constituents will generally also be applicable to inorganic constituents. NOTE 3 For ecotoxicological testing, eluates representing the release of both inorganic and organic contaminants are needed. In this document, ecotoxicological testing is also meant to include genotoxicological testing.