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This European Standard specifies a procedure for the estimation of biovolume of marine and freshwater phytoplankton taxa using inverted microscopy (Utermöhl technique according to EN 15204), in consideration of some heterotrophic protists (< 100 µm) that are not considered in routine zooplankton analysis and benthic microalgae, which can be found in pelagic water samples. This European Standard describes the necessary methods for measuring cell dimensions and for the calculation of cell or counting unit volumes to estimate the biovolume in phytoplankton samples. This shall be done using harmonized assignments of geometrical shapes to avoid errors.