SS-EN-ISO-15216-1 Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 1: Method for quantification (ISO 15216-1:2017)

SS-EN-ISO-15216-1 - 2017 EDITION - CURRENT


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ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR. This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.

To find similar documents by classification:

07.100.30 (Food microbiology Including microbiology of animal feeding stuffs Animal feeding stuffs, see 65.120 General methods of tests and analysis for food products, see 67.050)

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Designation Name

SS-EN ISO 15216-1:2017

Revision Level

2017 EDITION

Status

Current

Publication Date

April 6, 2017

Language(s)

English

Page Count

64

International Equivalent

EN ISO 15216-1:2017(IDT); ISO 15216-1:2017(IDT)