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This International Standard specifies a method using diethyl ether extraction for the determination of the unsaponifiable matter content of animal and vegetable fats and oils. This method is not applicable to waxes and, moreover, gives approximate results with certain fats of high unsaponifiable matter content, for example with fats derived from marine animals. A method given in ISO 18609 may be used when climatic conditions, or regulations, do not permit the use of diethyl ether.