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Standard Guide for Identification of Herpes Simplex Virus or its DNA
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Scope
1.1 This guide covers laboratory characterization procedures sufficient to identify purified specimens of HSV types 1 and 2 (HSV-1 and HSV-2) or HSV-1 DNA and HSV-2 DNA used in biotechnology. For cases in which identification of HSV DNA specimens is required, the characterization criteria of 6.2 and 6.3 of this guide are sufficient.
1.2 This guide does not cover the identification of HSV in HSV-infected host cells. To apply this guide to such a case, it would first be necessary to isolate the virus from such samples using standard techniques of HSV purification. This guide does not cover characterization of segments of HSV DNA or of vectors containing HSV DNA segments.
1.3 This guide does not cover the specific methodology used in the identification characterization. It does not address the question of degree of purity required for herpesvirus preparations: this would vary depending on the particular biotechnology use of the virus.
1.4 Warning--Laboratory work involving herpes simplex viruses can be hazardous to personnel. Precaution: Biosafety 2 level facilities are recommended (1). Safety guidelines shall be adhered to according to NCCLS M29-T2 and other recommendations (1).
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
Keywords
biotechnology; DNA; genetic examination; Herpes simplex virus (HSV); immunological evidence; molecular virology; virology; identification of herpes simplex virus (HSV) or its DNA, guide; ICS Number Code 07.100.10 (Medical microbiology)
To find similar documents by ASTM Volume:
11.06 (Biological Effects and Environmental Fate; Biotechnology)
To find similar documents by classification:
07.100.10 (Medical microbiology Laboratory medicine, see 11.100)
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Document Number
ASTM-E1286-00
Revision Level
2000 EDITION
Status
Superseded
Modification Type
Revision
Publication Date
Sept. 10, 2000
Document Type
Guide
Page Count
3 pages
Committee Number
E48.02