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Scope

1.1 This test method describes a procedure for determining the electrophoretic mobility of proteins of molecular weight greater than 10000 Daltons.

1.2 This test method uses automatic Electrophoretic Light Scattering (ELS) principles to determine the electrophoretic mobility.

1.3 The instrument simultaneously measures the Doppler shifts of scattered light at four different angles to determine the electrophoretic mobility distribution of protein particles. The mobility is expressed as [mu]m-cm/V-s (micron-centimeter/volt-second).

1.4 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Keywords

Collimated laser light; Doppler shifts; Electrophoretic light scattering (ELS); Lasers and laser applications; Light-scattering; Proteins; Zeta potential distribution; electrophoretic mobility of proteins (of molecular weight; ICS Number Code 07.080 (Biology. Botany. Zoology)

To find similar documents by ASTM Volume:

11.06 (Biological Effects and Environmental Fate; Biotechnology)

14.02 (General Test Methods; Forensic Psychophysiology; Forensic Sciences; Terminology; Conformity Assessment; Statistical Methods; Nanotechnology; Forensic Engineering; Manufacture of Pharmaceutical Products)

To find similar documents by classification:

07.080 (Biology. Botany. Zoology Including biotechnology)

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