ASTM-E1532 › Historical Revision Information
Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of the Bisbenzamide DNA-Binding Fluorochrome
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Scope
1.1 This practice covers procedures used for the detection of mycoplasma contamination by DNA staining.
1.2 This practice does not cover cultivation methods for the detection of mycoplasma or other methods such as biochemical detection, hybridization methodology, histochemical stains, or immunochemical methods.
1.3 This practice does not cover methods for the identification of mycoplasma organisms.
1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.5 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
Keywords
Bisbenzamide fluorochrome stain; Cell culture; Contamination-environmental; Cultivation; DNA; Indicator cells; Microbiological examination; Mycoplasma; mycoplasma in cell culture-indirect detection, by DNA binding with; bisbenzamide fluorochrome stain, practice; ICS Number Code 07.100.10 (Medical microbiology)
To find similar documents by ASTM Volume:
11.06 (Biological Effects and Environmental Fate; Biotechnology)
To find similar documents by classification:
07.100.10 (Medical microbiology Laboratory medicine, see 11.100)
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Document Number
ASTM-E1532-00
Revision Level
2000 EDITION
Status
Superseded
Modification Type
Revision
Publication Date
May 10, 2000
Document Type
Practice
Page Count
2 pages
Committee Number
E48.02