ASTM-E2525 › Historical Revision Information
Standard Test Method for Evaluation of the Effect of Nanoparticulate Materials on the Formation of Mouse Granulocyte-Macrophage Colonies (Withdrawn 2022)
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Scope
1.1 This test method provides a protocol for quantitative analysis of the effect of nanoparticulate materials in physiologic solution on granulocyte-macrophage colony-forming units.
1.2 This test method employs murine bone marrow hematopoietic stem cells which proliferate and differentiate to form discrete cell clusters or colonies which are counted.
1.3 This test method is part of the in vitro preclinical characterization cascade for nanoparticulate materials for systemic administration in medical applications.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
Significance and Use
Stem cells of hematopoietic origin are pluripotential and may be particularly sensitive to the effects of stimulation by nanoparticulate materials.
The effect of particles on macrophage responses has an extensive history and can be assessed by Practice F 1903
Keywords
bone marrow; CFU-GM; clonogenic assay; granulocyte; macrophage; myelosuppression; nanoparticles; preclinical development; stem cells; ICS Number Code 07.100.10 (Medical microbiology)
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07.100.10 (Medical microbiology Laboratory medicine, see 11.100)
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Document Number
ASTM-E2525
Revision Level
WITHDRAWN
Status
Cancelled
Modification Type
New
Publication Date
April 19, 2022
Document Type
Test Method
Committee Number
E56.03