ISO-15216-1 › Historical Revision Information
AMENDMENT 1, Part 1: Method for quantification

ISO-15216-1 - FOR 1ST EDITION AMENDMENT 1 SEE - CURRENT
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ISO/TS 15216-1:2013 describes a method for quantification of levels of HAV and NoV genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.

This approach is also relevant for detection of the target viruses on fomites, or of other human viruses in foodstuffs, on food surfaces or on fomites following appropriate validation and using target-specific primer and probe sets.

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07.100.30 (Food microbiology Including microbiology of animal feeding stuffs Animal feeding stuffs, see 65.120 General methods of tests and analysis for food products, see 67.050)

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Document Number

ISO-15216-1

Revision Level

FOR 1ST EDITION AMENDMENT 1 SEE

Status

Current

Publication Date

April 1, 2021

Committee Number

ISO/TC 34/SC 9