ISO-8128-2 › Apple juice, apple juice concentrates and drinks containing apple juice -- Determination of patulin content -- Part 2: Method using thin-layer chromatography

ISO-8128-2 - 1993 EDITION - CURRENT


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The principle of the method specified is extraction of patulin in a mixture of ethyl acetate and chloroform (3:2 by volume), filtration of the extract on a silica-gel column and qualitative and quantitative determination by means of two-directional thin-layer chromatography. The spots are developed using a 3-methyl-2-benzothiazoline hydrazone (MBTH) hydrochloride solution. The limit of detection is 25 µg/l, based on 50 ml of ready-to-drink apple juice. The more precise method of analysis is HPLC.

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67.160.20 (Non-alcoholic beverages Including juices, nectars, mineral waters, lemonades, root beer, cola drinks, etc. Tea, coffee, cocoa, see 67.140)

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Document Number

ISO 8128-2:1993

Revision Level

1993 EDITION

Status

Current

Publication Date

Jan. 1, 1993

Committee Number

ISO TC 34/SC 3