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This International Standard provides guidelines on the quantitative collection and processing of subtidal soft-bottom macrofaunal samples in marine waters. This International Standard encompasses: -- development of the sampling programme; -- requirements for sampling equipment; -- sampling and sample treatment in the field; -- sorting and species identification; -- storage of collected and processed material. This International Standard does not specifically address the following, although some elements may be applicable: -- bioassay sub-sampling; -- deep water (> 750 m) or offshore sampling; -- in situ faunal studies, e.g. recolonisation assays; -- nonbenthic organisms caught in the sampling device; -- estuarine sampling; -- intertidal sampling; -- meiofaunal sampling and analysis [3]; -- sampling by dredge and sledge; -- Self-Contained Underwater Breathing Apparatus (SCUBA) sampling; -- statistical design. Accuracy of position fixing is determined by the geographical area, equipment used and survey objective.