ASTM-D5245 Historical Revision Information
Standard Practice for Cleaning Laboratory Glassware, Plasticware, and Equipment Used in Microbiological Analyses

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Standard Practice for Cleaning Laboratory Glassware, Plasticware, and Equipment Used in Microbiological Analyses


1.1 In microbiology, clean glassware is crucial to ensure valid results. Previously used or new glassware must be thoroughly cleaned. Laboratory ware and equipment that are not chemically clean are responsible for considerable losses in personnel time and supplies in many laboratories. These losses may occur as down time when experiments clearly have been adversely affected and as invalid data that are often attributed to experimental error. Chemical contaminants that adversely affect experimental results are not always easily detected. This practice describes the procedures for producing chemically clean glassware.

1.2 The values stated in SI units are to be regarded as the standard.

1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific precautions, see Section 5, 7.3.1, and Note 1 and Note 2.

Significance and Use

This practice provides uniform guidance for cleaning the laboratory glassware, plasticware, and equipment used in routine microbiological analyses. However, tests that are extremely sensitive to toxic agents (such as virus assays) may require more stringent cleaning practices.


acid cleaning; alkali-cleaning; cleaning glassware; cleaning plasticware; glasswasher; laboratory waste disposal; ICS Number Code 07.100.01 (Microbiology in general)

To find similar documents by ASTM Volume:

11.02 (Water (II))

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07.100.01 (Microbiology in general)

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Document Number


Revision Level

1992 R12 EDITION



Modification Type


Publication Date

Sept. 1, 2012

Document Type


Page Count

5 pages

Committee Number