ASTM-D5259 › Historical Revision Information
Standard Test Method for Isolation and Enumeration of Enterococci from Water by the Membrane Filter Procedure
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Scope
1.1 This test method covers a membrane filter (MF) procedure for the detection and enumeration of the enterococci bacteria in water. The enterococci, which include Entero-coccus faecalis (E. faecalis), E. faecium, and their varieties are commonly found in the feces of humans and other warm-blooded animals. Although some strains are ubiquitous and not related to fecal pollution, enterococci in water are an indication of fecal pollution and the possible presence of enteric pathogens. These bacteria are found in water and wastewater in a wide range of densities. The detection limit is one colony forming unit (CFU)/volume filtered.
1.2 This test method has been used successfully with temperate fresh and marine ambient waters, and wastewaters. It is the user's responsibility to ensure the validity of this test method for waters of untested types.
1.3 The values stated in SI units are to be regarded as the standard.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific hazard statements, see Section 9.
Keywords
enterococci; Enterococcus faecalis, Enterococcus faecium, Enterococcus avium, fecal pollution; m-E agar; two-step membrane filter procedures; ICS Number Code 13.060.70 (Examination of water for biological properties)
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13.060.70 (Examination of biological properties of water Microbiology of water, see 07.100.20)
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Document Number
ASTM-D5259-92(2000)
Revision Level
1992 R00 EDITION
Status
Superseded
Modification Type
Reapproval
Publication Date
June 15, 1992
Document Type
Test Method
Page Count
6 pages
Committee Number
D19.24