ASTM-D5465 Historical Revision Information
Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods

ASTM-D5465 - 1993 R12 EDITION - SUPERSEDED
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Standard Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
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Scope

1.1 These practices cover recommended procedures for counting colonies and reporting colony-forming units (CFU) on membrane filters (MF) and standard pour and spread plates.

1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Significance and Use

These practices provide a uniform set of counting, calculating, and reporting procedures for ASTM test methods in microbiology.

Section
ACounting Colonies on Membrane Filters4
BCounting Colonies on Pour Plates5
CCounting Colonies on Spread Plates6

The counting rules provide a best attainable estimate of microorganisms in the sample, since the samples cannot be held and reanalyzed at a later date.

Keywords

calculating results; counting colonies; membrane filter methods; pour plate methods; reporting results; rounding off; significant figures; spread plate methods; verification of counts; ICS Number Code 07.100.20 (Microbiology of water)

To find similar documents by ASTM Volume:

11.02 (Water (II))

To find similar documents by classification:

07.100.20 (Microbiology of water Examination of biological properties of water, see 13.060.70)

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Document Number

ASTM-D5465-93(2012)

Revision Level

1993 R12 EDITION

Status

Superseded

Modification Type

Reapproval

Publication Date

Sept. 1, 2012

Document Type

Practice

Page Count

4 pages

Committee Number

D19.24