ISO-21569-2 › Molecular biomarker analysis - Methods of analysis for the detection of genetically modified organisms and derived products - Part 2: Construct-specific real-time PCR method for detection of event FP967 in linseed and linseed products

ISO-21569-2 - 2ND EDITION - CURRENT -- See the following: ISO-21569-AM1
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This document specifies a procedure for the detection of a DNA sequence present in a genetically modified linseed (Linum usitatissimum) line (event FP967, also named as â..CDC Triffidâ..). For this purpose, extracted DNA is used in a real-time PCR and the genetic modification (GM) is specifically detected by amplification of a 105 bp DNA sequence representing the transition between the nopaline synthase gene terminator (Tnos) from Agrobacterium tumefaciens and the dihydrofolate reductase gene (dfrA1) from a Class 1 integron of Escherichia coli.

The method described is applicable for the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix for the purpose of analysis.

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67.050 (General methods of tests and analysis for food products Food microbiology, see 07.100.30 Sensory analysis, see 67.240)

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Document Number

ISO/TS 21569-2:2021

Revision Level

2ND EDITION

Status

Current

Publication Date

July 1, 2021

Committee Number

ISO/TC 34/SC 16